Fig. 2.

INT-M encodes an APETALA2 transcription factor. (A) RNA-seq revealed a ∼10-Mb overlapping introgression region of the allelic int-m.1a and int-m.85. Functional annotation of variants within this introgression region identified a barley ortholog (HvAP2L-H5) of maize INDETERMINATE SPIKELET1 (IDS1) and wheat AP2L-A5 as the candidate gene. (B) Maximum likelihood phylogenetic tree of INT-M/IDS1 genes; bootstrap values of >50 are shown above branches. (C) INT-M gene model with position of mutations in 24 allelic mutants termed dub1 (SI Appendix, Table S1). (D) Two exemplary allelic dub1 mutants (M21 and M22 in Bonus background) produce determinate inflorescences and desuppressed RSMs (circled by dashed lines) in M21.