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. 2021 Feb 17;118(8):e2017213118. doi: 10.1073/pnas.2017213118

Fig. 1.

Fig. 1.

Severe neuronal damage in gray matter of Type-B EAE mice. (A) Schematic for two EAE mouse models. (B) Daily EAE disease scores of Type-A and Type-B EAE mice up to 60 dpi (Type-A EAE: n = 6; Type-B EAE: n = 6). (C) Representative brightfield images of Golgi-Cox–stained lumbar spinal cord hemisections (Left) and quantification (Right) of percentage area stained over ROI area marked by red dotted lines (GM, gray matter) at 28 dpi (n = 6 per condition). Data are a compilation of two independent experiments. (D) Size of Golgi-Cox+ cells in vGM (n = 3 per condition). (E) Representative 3D images of Golgi-Cox–stained neuron dendrites in vGM (Left). Quantitative analysis of dendritic spine density (Right) at 28 dpi (n = 3). Statistical analysis: two-tailed Student’s t test (B), Mann–Whitney u test (C–E). *P < 0.05, ns, not significant. Schematic cartoons in (A) are credited to https://biorender.com/.