Fig. 4.

Cytotoxicity of compounds 33-11/KH-4-43 against a panel of tumor lines. (A) The ability of 33-11 to induce apoptosis was measured by Annexin V flow cytometry as described in SI Appendix, Methods. EC₅₀/EC₉₀ for each cell line is indicated. Note that EC₅₀ or EC₉₀ valued at 100 µM is the upper limit, representing those with EC₅₀ or EC₉₀ ≧100 µM. (B) Toxic response of a panel of cancer cells to 33-11/KH-4-43, as determined by viability assay (CellTiter-Glo). Note that mouse pancreatic cancer cell lines K8484 and DT8082 were isolated from KPC mice (38); the rest (34 in total) are all human cancer cell lines. (C) Washout. MV4-11 cells at 10⁵ cells per milliliter were treated with compound at the concentrations indicated. At specified hours post compound treatment, the cells were washed once with fresh complete media without compound and allowed to grow for an additional 48 h followed by a viability test as in B. For B and C, the graph shows the quantification of three independent experiments with error bars indicating experimental variations.