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. 2021 Mar 1;40:86. doi: 10.1186/s13046-021-01890-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — TRIP13 was correlated with poor prognosis and progression of pCCA. a TRIP13 expression in biliary cell lines. Up: Expression of TRIP13 in HiBEpiC, iCCA cell lines, pCCA cell lines and gallbladder cancer cells. Bottom: the quantification of the TRIP13 bands in WB. b TRIP13 mRNA levels in 18 pairs of pCCAs and adjacent normal bile duct tissues were detected with qRT-PCR, and showed as log (TRIP13^Tumor/TRIP13^Non-tumor). c TRIP13 expression was detected in four pairs of pCCA tissues and adjacent normal duct tissues with WB (up), and the bands were quantified (bottom). d The prognostic significance of TRIP13 expression (up), and co-expression of TRIP13 and HMGA1(bottom) were analyzed with survival analysis by log-rank test. e HMGA1 was knocked down in QBC-939 cells and overexpressed in FRH-0201 cells; cell proliferation was detected with CCK8 assays within 4 days of culture. f,g Migration and invasion of QBC-939 and FRH-0201 cells, as detected by wound healing assay (f) or transwell assay(g) after silencing or overexpressing HMGA1. h 7-day sphere formation assays were performed to determine the effects of TRIP13 on stem cell characteristics of pCCA cells. i, j Effect of TRIP13 on the EMT in pCCA cells was evaluated by WB after silencing (i) or overexpressing (j) TRIP13 expression. *, ** and *** represents P < 0.05, P < 0.01 and P < 0.001, compared with the corresponding control group, analyzed with the T-test (c,f-h) or one-way ANOVA (e). Data were from three independent experiments and shown as means ± S.E.M.