Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 1;40:86. doi: 10.1186/s13046-021-01890-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — FBXW7 suppressed TRIP13-induced stemness and EMT. a TRIP13 knockdown decreased FBXW7 expression in QBC-939 and FRH-0201 cells. b Luciferase assays showed that TRIP3 inhibited FBXW7 transcription in QBC-939 and FRH-0201. c Silencing TRIP13 suppressed pCCA proliferation and knocking down FBXW7 had contrary effects. d,e Transwell assay showed that silencing FBXW7 promoted the migration(d) and invasion(e) which was decreased by TRIP13 knockdown. f,g QBC-939 and FRH-0201 were transfected with shTRIP13 and/or siFBXW7. The biomarkers of stemness (CD44 and OCT4)(f) and 7-day sphere formation assay(g) were performed. (H) EMT biomarkers were detected after regulating TRIP13 and/or FBXW7 expression in QBC939(left) or FRH0201(right). In (b,d,e,g), ** and *** represents P < 0.01 and < 0.001, compared with corresponding control group or indicated groups. In (b,d), the P value was calculated with T-test(b,d,g) or one-way ANOVA(c). Analyzed data were from three independent experiments, and each subgroup was performed at least in triplicate