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. 2021 Mar 2;54:7. doi: 10.1186/s40659-021-00330-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effect of lotus leaves flavonoids (LLF) on the apoptosis marker Annexin-V over A549 lung cancer cells. A549 cells were exposed to LLF at different concentrations and the fluorescence intensity after Annexin-V stain was measured by flow cytometry. a Control: untreated A549 cells; b A549 cells treated with LLF at 125 µg/mL for 48 h; c A549 cells treated with LLF at 250 µg/mL for 48 h; d A549 cells treated with LLF at 500 µg/mL for 48 h. Frequency histogram represents the median fluorescence intensity (MFI) against the cell count. The increase in the MFI represents an increase in the number of the cells positive for the apoptosis mark Annexin-V (n = 6)