Fig. 5.

The target association of miR-650 and CNP. a The targets of miR-650 were predicted by miRDB, miRwalk, starBase, and Tarbase. b The levels of the predicted targets were detected by qRT-PCR in cells transfected with miR-NC or miR-650 mimic. c CNP and RAC1 protein levels were detected via Western blotting in cells transfected with miR-NC or miR-650 mimic. d The target sites of miR-650 and CNP. e Luciferase activity was measured in cells transfected with WT-CNP 3′UTR or MUT-CNP 3′UTR and miR-NC or miR-650 mimic. f, g CNP level was measured via qRT-PCR and Western blotting in RAFLSs transfected with miR-NC, miR-650 mimic, anti-NC, or anti-miR-650. h, i CNP level was determined by qRT-PCR and Western blotting in RAFLSs with transfection of vector, circ-AFF2 overexpression vector, circ-AFF2 overexpression vector + miR-NC or miR-650 mimic, si-NC, si-circ-AFF2, and si-circ-AFF2 + anti-NC or anti-miR-650. *P < 0.05, ** P< 0.01, *** P< 0.001