Fig. 1.

RPA and Srs2 show antagonistic relationship. (A, Upper) Schematic of the Rfa1 protein domains and rfa1 mutant alleles. (Lower) Structure of the Ustilago maydis RPA in complex with ssDNA (black sticks) (23). The Zn²⁺ finger region is boxed red. (B) rfa1 mutants’ sensitivity toward CPT and MMS is suppressed by srs2Δ. A 10-fold serial dilution of cells of the indicated genotypes were spotted and growth was assessed after incubation at 30 °C for 3 d. Dashed lines indicate removal of superfluous rows.