Table 1. Echocardiographic data from 2-mo-old WT and Metrnβ−/− mice after ISO-induced hypertrophy.
| CT | ISO | ||||
|---|---|---|---|---|---|
| WT | Metrnβ−/− | WT | Metrnβ−/− | P value | |
| HW/TL (mg/mm) | 6.88 ± 0.16 | 7.32 ± 0.38 | 8.75 ± 0.33*** | 8.59 ± 0.18** | **** |
| HW/BW (mg/g) | 4.10 ± 0.11 | 4.35 ± 0.12 | 5.20 ± 0.21*** | 5.05 ± 0.20* | **** |
| IVSd (mm) | 0.57 ± 0.01 | 0.56 ± 0.01 | 0.60 ± 0.02 | 0.69 ± 0.01****,### | ****, #, $$$ |
| LVPWd (mm) | 0.69 ± 0.03 | 0.67 ± 0.02 | 0.94 ± 0.08*** | 0.78 ± 0.02# | ***, # |
| LVIDd (mm) | 4.21 ± 0.08 | 4.05 ± 0.13 | 4.14 ± 0.06 | 4.15 ± 0.04 | |
| IVSs (mm) | 0.92 ± 0.03 | 0.88 ± 0.05 | 1.05 ± 0.06 | 1.22 ± 0.03***,# | ****, $ |
| LVPWs (mm) | 0.93 ± 0.03 | 0.80 ± 0.02 | 1.23 ± 0.07*** | 1.02 ± 0.03**,## | ****, ## |
| LVIDs (mm) | 3.08 ± 0.07 | 3.27 ± 0.04 | 2.96 ± 0.07 | 2.97 ± 0.03** | ** |
| EF (%) | 59.3 ± 1.1 | 50.8 ± 2.1## | 61.2 ± 1.2 | 62.3 ± 1.1**** | ***, #, $$ |
| FS (%) | 27.2 ± 0.6 | 23.0 ± 0.7## | 27.8 ± 0.8 | 28.8 ± 0.7**** | ***, #, $$ |
| EDV (mm3) | 79.4 ± 3.5 | 77.7 ± 3.7 | 76.1 ± 2.7 | 76.28 ± 1.8 | |
| ESV (mm3) | 37.5 ± 2.1 | 43.1 ± 1.2 | 34.1 ± 2.1 | 34.2 ± 0.9** | ** |
| Heart rate | 308.2 ± 25.9 | 358.6 ± 28.9 | 474.6 ± 37.3*** | 511.8 ± 11.22** | **** |
| LVm (mg) | 93.3 ± 2.4 | 84.2 ± 2.9 | 126.5 ± 11.3** | 110.3 ± 4.0* | ** |
| Aortic peak (m/s) | 1.11 ± 0.07 | 1.24 ± 0.06 | 1.39 ± 0.09* | 1.54 ± 0.07* | ** |
| VTI (cm) | 6.43 ± 0.53 | 6.56 ± 0.29 | 5.30 ± 0.65 | 5.70 ± 0.42 | |
| E peak (m/s) | 1.09 ± 0.10 | 0.98 ± 0.10 | 1.18 ± 0.05 | 1.26 ± 0.06 | |
| Mitral deceleration (ms) | 36.0 ± 2.7 | 30.2 ± 0.6 | 31.0 ± 1.8 | 27.3 ± 1.3 | *, # |
| Aortic diameter (mm) | 1.12 ± 0.02 | 1.20 ± 0.07 | 1.36 ± 0.01** | 1.43 ± 0.07* | *** |
All measurements are expressed as mean ± SEM; n = 6 mice/group. Data were analyzed by two-way ANOVA, where *, P < 0.05, **, P < 0.001, ***, P < 0.0001, ****, P < 0.00001 for ISO effect; #, P < 0.05, ##, P < 0.001, ###, P < 0.0001 for genotype effect; $, P < 0.05, $$, P < 0.001, $$$, P < 0.0001 for interaction effect. CT, control; d, after diastole; EDV, end diastolic volume; HW/BW, heart weight/body weight ratio; s, after systole.