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. 2021 Feb 20;19(2):117. doi: 10.3390/md19020117

Figure 6.

Figure 6

PNSA inhibits the migration, invasion, and EMT-related signaling pathway proteins of JIMT-1 cells. (A) Inhibitory effect of PNSA on the migration of JIMT-1 cells. Representative images of the wound healing assay performed with JIMT-1 cells treated with PNSA (0.25, 0.5, 1, 2 μM), the migrated ratio was calculated (right panel). (B) Inhibitory effect of PNSA on the invasion of JIMT-1 cells. JIMT-1 cells were incubated with PNSA (0.25, 0.5, 1, 2 μM) for 12 h through a Matrigel-coated Boyden Chamber, the invasion cells were counted (right panel). (C) Effect of PNSA on cell viability of JIMT-1. JIMT-1 cells were incubated with the indicated concentrations of PNSA for 12 h and cell viability was measured using SRB assay. (D) Effects of PNSA on the expressions and activations of signaling molecules related to EMT. Protein expressions and activations were analyzed by Western blotting. GAPDH was used as a loading control. The bar graph represents the average ± SD of at least three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant (relative to DMSO-treated cells).