Figure 2.

Workflow for identification of vaccine candidates and vaccine formulation using high throughput omics techniques. The material of interest (DNA, RNA or protein) can be extracted from the desired parasite or from-infected host fish tissue. The isolated material serves as sample for next generation sequence analysis (for DNA and RNA) and spectrophotometry and Edman sequencing (for protein). The obtained sequences can be subjected to different bioinformatic tools for analysis and functional annotation of parasite molecules. Based on molecular function and biological pathway analysis, the immunogenic targets (capable of eliciting host immune response) can be selected and their protective epitopes predicted. These molecules can then be produced on a large scale and combined with suitable adjuvants to form vaccine. Subsequently, the vaccine thus produced can be used for conducting trials in suitable fish and its efficacy and appropriate route of administration can be determined.