Table 1.
(S)-Linalool production in test-tube cultivation
| Host strain | Plasmid | (S)-linalool (g/L)a | Consumed glucose (g/L)a | Yield (%)b |
|---|---|---|---|---|
| SWITCH-PphoC Δgcd | pACYC177 | NDc | 43 ± 3.3 | – |
| SWITCH-PphoC Δgcd | pAaLINS-ispA* | 3.4 ± 0.2 | 60 ± 0.0 | 5.6 ± 0.3 |
| SWITCH-PphoC Δgcd | pBLAAaLINS-ispA* | 3.0 ± 0.0 | 60 ± 0.0 | 5.0 ± 0.0 |
| IP03 | pAaLINS-ispA* | 0.8 ± 0.0 | 43 ± 0.6 | 1.9 ± 0.0 |
| IP03 | pBLAAaLINS-ispA* | 4.0 ± 0.2 | 60 ± 0.0 | 6.6 ± 0.4 |
| IP04 | pACYC177 | ND | 38 ± 1.7 | – |
| IP04 | pAaLINS-ispA* | 1.0 ± 0.0 | 36 ± 0.6 | 2.8 ± 0.1 |
| IP04 | pBLAAaLINS-ispA* | 4.7 ± 0.3 | 60 ± 0.0 | 7.9 ± 0.2 |
All strains were cultivated for 48 h under biphasic fermentation using isopropyl myristate. Data are expressed as the mean ± SD of at least three biological replicates
aGlucose and (S)-linalool concentrations are represented by dividing the total amounts by the volume of aqueous culture
bYield was calculated as grams of product per grams of consumed glucose and is expressed as a percentage. Carbon sources contained in 2 g/L of Bacto yeast extract was not considered in this calculation
cND, Not detected