Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 4;11:598884. doi: 10.3389/fimmu.2020.598884

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Shao, Meng, Liu, Xu, Wang, Hu, Hou and Ho

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effect of RIG-I knockout on poly(dA:dT)-mediated HSV-2 inhibition. End1/E6E7 control cells (V2 control) and RIG-I knockout End1/E6E7 cells (RIG-I^−/−) were transfected with or without poly(dA:dT) (0.5μg/ml) for 24h prior to HSV-2 infection (MOI = 0.001). (A, B) Total DNAs extracted from cells (intracellular) and culture supernatant (Extracellular) were measured by the real-time PCR for HSV-2 gD expression. (C) Total cellular proteins were collected and subjected to Western blot with antibodies against HSV-2 gB, HSV-2 gD, RIG-I, and GAPDH. The results are the mean ± SD of three independent experiments. Asterisks indicate that the differences between the indicated groups are statistically significant (**P < 0.01).