Table 1.
Test strains, growth conditions for initial cultivation of the lyophilisates, and CFU of the diluted straines used for inoculation of the blood culture bottles
Strain | ATCC No. | Growth conditions | CFU/100 µL |
---|---|---|---|
Aerobic bacteria | |||
S. aureus1 | ATCC 25923 | CBA, overnight | 45 |
P. aeruginosa1 | ATCC 9027 | CBA, overnight | 41 |
B. subtilis1 | ATCC 6633 | CBA, overnight | 32 |
S. epidermidis | ATCC 12228 | CBA, overnight | 21 |
E. faecalis | ATCC 51299 | CBA, overnight | 47 |
Anaerobic bacteria | |||
C. sporogenes1 | ATCC 19404 | CBA, 3 days | 26 |
C. acnes | ATCC 6919 | CBA, 5 days | 54 |
Fungi | |||
C. albicans1 | ATCC 10231 | SDA, overnight | 34 |
A. brasiliensis1 | ATCC 16404 | SDA, 9 days | 10 |
Recommended for validation by the Ph. Eur. CFU, colony-forming units.