Table 2.

Preparation of test bottles.

Test bottle	BD BACTECTM bottle	Medium	Volume, mL	Enzyme1, mL	Test strain, µL
CM I/II aerobic	Plus Aerobic/F	CM I/CM II	8.5±0.5	1	100
CM I/II anaerobic	Plus Anaerobic/F	CM I/CM II	8.5±0.5	1	100
FH aerobic	Standard Aerobic/F	FH	9.5±0.5	−	100
FH anaerobic	Standard Anaerobic/F	FH	6.5±0.5	−	100
Negative controls
(−) control aerobic CM I/II	Plus Aerobic/F	CM I/CM II/saline solution	8.5±0.5	1	−
(−) control anaerobic CM I/II	Plus Anaerobic/F	CM I/CM II/	8.5±0.5	1	−
		saline solution
(−) control aerobic FH	Standard Aerobic/F	FH/saline solution	9.5±0.5	−	−
(−) control anaerobic FH	Standard Anaerobic/F	FH/saline solution	6.5±0.5	−	−
Positive controls
(+) control aerobic CM I/II	Plus Aerobic/F	saline solution	8.5±0.5	1	100
(+) control anaerobic CM I/II	Plus Anaerobic/F	saline solution	8.5±0.5	1	100
(+) control aerobic FH	Standard Aerobic/F	saline solution	9.5±0.5	−	100
(+) control anaerobic FH	Standard Anaerobic/F	saline solution	6.5±0.5	−	100

The test bottles are named after their injected medium or as controls for the corresponding medium. Culture media I and II contain antibiotics and are injected in aerobic and anaerobic BD BACTEC^TM Plus bottles and penicillinase is added. The femoral head medium is injected in aerobic and anaerobic standard bottles without resins. The manufacturer's recommendations for the addition of test media are: 8–10 mL for Plus Aerobic/F, Plus Anaerobic/F, and Standard Aerobic/F bottles and 5–7 mL for Standard Anaerobic/F bottles. (−) negative; (+) positive.

¹Penicillinase 1 mL inactivates 1 × 10⁷ IU penicillin G.