Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 2;184(8):2212–2228.e12. doi: 10.1016/j.cell.2021.02.053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Elsevier Inc.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Figure S4 — Characterization of subcellular localization of the wild-type and mutant ACE2, related to Figure 6

(A) WB analysis showing expression levels of cACE2 and sACE2 in 293T cells transfected with a plasmid encoding wild-type ACE2 (lane 1), GFP-tagged mutant ACE2 lacking the transmembrane domain (GFP-ACE2ΔTM) (lane 2), or GFP (lane 3) prior to SARS-CoV-2 infection.

(B) IFA of SARS-CoV-2 NP (green) in 293T cells transfected with wild-type ACE2 (left) and mutant GFP-ACE2ΔTM (right). Cells were counterstained with DAPI to label nuclei (blue). Scale bars represent 20 μm.