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. 2021 Feb 25;13:1967–1979. doi: 10.2147/CMAR.S287386

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© 2021 Li et al.

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GJB2 is upregulated and positively associated with CAR10 expression in NSCLC. (A) Diagram of screening the DEGs in the GEO datasets, GSE19188, GSE30219 and GSE118370 using the GEO2R online software. (B) Prognostic value of GJB2 in 1145 patients with NSCLC was determined using the Kaplan-Meier Plotter tool (https://kmplot.com/analysis/). GJB2 expression in NSCLC and normal lung tissues or ANTs was analyzed in (C) GSE19188, (D) GSE30219 and (E) GSE118370. (F) Expression of GJB2 in patients at different stages of NSCLC was detected using immunohistochemical analysis. *P=0.0106, **P=0.0029, ****P<0.0001. Magnification, x400. Scale bar, 50 μm. (G) GJB2 expression in a normal human bronchial epithelial cell line (16HBE) and in four NSCLC cell lines (A549, H1975, SPCA1 and H1299) was determined using reverse transcription-quantitative PCR. ***P<0.001. Correlation between CAR10 and GJB2 expression in the (H) GSE19188, (I) GSE32219 and (J) GSE118370 GEO datasets. (J) was assessed using Pearson’s correlation analysis. The data are presented as the mean ± SD from three independent experiments.

Abbreviations: GJB2, gap junction protein beta 2; CAR10, chromatin-associated RNA Intergenic 10; NSCLC, non-small cell lung cancer; DEG, differentially expressed gene; GEO, Gene Expression Omnibus; ANT, adjacent normal tissue.