Figure 7.

CAR10 promotes A549 and H1299 cell migration and invasion by regulating the miR-892a/GJB2 axis. CAR10 upregulation enhanced GJB2 protein expression in the (A) A549 and (B) H1299 cells. The facilitative effect was dismissed when the binding sites were mutated, and GJB2 suppression also reversed the promotive effect of oeCAR10. miR-892a inhibition restored the expression of GJB2. Not significant, P>0.05, and **P<0.01. Transwell and Matrigel assays were performed in the (C) A549 and (D) H1299 cells using the aforementioned treatments. Metastatic abilities of the A549 and H1299 cell lines were altered in accordance with GJB2 protein expression level, as aforementioned. Not significant, P>0.05 and **P<0.01. The data are presented as the mean ± SD from three independent experiments.

Abbreviations: CAR10, chromatin-associated RNA Intergenic 10; miR, microRNA; GJB2, gap junction protein beta 2.