Table 2.

Activity of compounds 1–3 on L. donovani intracellular amastigotes, promastigotes and THP-1 cells

Compounds	IC50 (μM) Intracellular amastigotes	IC50 (μM) Promastigotes	CC50 (μM) THP-1*	Selectivity Index**
1	2.32 ± 0.13	0.118 ± 0.011	11.48 ± 1.11	4.94
2	0.091 ± 0.010	0.034 ± 0.004	6.40 ± 0.46	70.32
3	0.073 ± 0.010	0.046 ± 0.009	6.07 ± 0.82	83.15
Doxorubicin	---	---	0.58 ± 0.04	---
Miltefosine	5.33 ± 0.74	4.98 ± 0.37	60.73 ± 2.21	11.39

Data are shown as mean ± SD (n = 3 biological replicates)

^*THP-1 human leukemia macrophages (host cells of L. donovani)

^**Selectivity index = CC₅₀ THP-1/IC₅₀ intracellular amastigotes