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. 2021 Feb 20;10(2):453. doi: 10.3390/cells10020453

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Choline transporter mRNA and protein expression in human neural stem cells (hNSCs). (A) Quantitative real-time polymerase chain reaction (qPCR) analysis of choline transporter-like proteins (CTL1-5), high-affinity choline transporter 1 (CHT1), and poly-specific organic cation transporters (OCT1-3). The relative mRNA expression is shown as a ratio of the target mRNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Each value shows the mean ± standard deviation (SD) of three independent experiments. BLQ—below the lower limit of quantification. (B) Western blot analysis of CTL1 and CTL2.