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. 2021 Feb 20;10(2):453. doi: 10.3390/cells10020453

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of extracellular choline uptake inhibition by choline uptake inhibitor, HC-3, on cellular activities in hNSCs. (A) hNSCs proliferation at 5 days of cultivation in various HC-3 concentrations. hNSCs were seeded at 5 × 10⁴ cells/well on 48-multiwell plates. The results are presented as the percentage of day 1. (B) The hNSCs viability at 3 days of cultivation in various HC-3 concentrations. hNSCs were seeded at 5 × 10⁴ cells/well on 24-multiwell plates. The results are presented as a percentage of the 0 μM HC-3 group. (C) hNSCs Caspase-3/7 activity at 3 days of cultivation in various degrees of HC-3 concentrations. hNSCs were seeded at 5 × 10⁴ cells/well on 24-multiwell plates. The results are presented as the percentage of the 0 μM HC-3 group. Each value shows the mean ± SD of four independent experiments. * p < 0.05, ** p < 0.01 and **** p < 0.0001 denote statistical significance vs. the 0 μM HC-3 group using Dunnett multiple comparison test.