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. 2021 Feb 20;10(2):453. doi: 10.3390/cells10020453

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of extracellular choline uptake inhibition on neurite outgrowth in hNSCs. (A) Cultivation in the control differentiation medium (0 μM HC-3) for 7 days. Staining shows microtubule-associated protein 2 (MAP2) (red) and DAPI (blue). Scale bar: 200 μm. (B) Cultivation in the differentiation medium with 250 μM HC-3 for 7 days. Staining shows MAP2 (red) and DAPI (blue). Scale bar: 200 μm. (C) Comparison of the neurite length in various HC-3 concentrations medium. The neurite length was measured in randomly chosen cells (30 cells) by tracing individual neurites. **** p < 0.0001 denotes statistical significance vs. 0 μM HC-3 group using Dunnett multiple comparison test. ns means not significant.