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. 2021 Feb 20;22(4):2098. doi: 10.3390/ijms22042098

Figure 2.

Figure 2

Anti-apoptotic effect of EA in primary neurons. (A) Representative images showed MPP+-induced neuronal cell death (white circle) and that pretreatment with 100 µM EA had a protective effect. Scale bar = 100 µm. (B) MTT assay confirmed that EA protected cells from MPP+-induced cell death. Values are means ± SEs (n = 8). *** p < 0.001 vs. naïve controls and ### p < 0.001 vs. MPP+-treated controls (the analysis was performed using ANOVA with Fisher’s PLSD procedure). (C) Western blot analysis confirmed that EA downregulated apoptotic markers in MPP+ treated primary neurons. (D–F) Bar graphs of fold changes of western blot. More than three independent experiments were performed and values are means ± SEs (n = 3–5). ** p, *** p < 0.01, 0.001 vs. naïve controls and # p, ## p < 0.05, 0.01 vs. MPP+-treated controls (the analysis was performed using ANOVA with Fisher’s PLSD procedure).