Table 2.
List of primers for expression of target genes used in the study.
| Gene Name/Genotype | Locus/ SALK |
Forward Primer (5′->3′) | Reverse Primer (5′->3′) | Gene Name |
|---|---|---|---|---|
| atbzip62 | SALK_053908C | TGGCACTTTTAACTTTGTGCC | TACGTTTCCATCGAGTGAACC | Arabidopsis bzip62 loss of function mutant |
| Drought responsive gene in Arabidopsis | ||||
| AtbZIP62 | AT1G19490 | CATCGAGTTGTTGCTCGTCG | AAATCCGCCAATGCTTCTGC | Basic-leucine zipper transcription factor encoding gene 62 |
| Genes involved in strigolactone biosynthesis pathway and controlling shoot branching (bud outgrowth) | ||||
| AtMAX1 | AT2G26170 | TGGTCACTTGCCCTTGATGG | GGTTGCCTCCCCATCTGAAA | More axillary branching 1 gene |
| AtMAX2 | AT2G42620 | CCGAGCCAGAGTTTGGGTTA | GTGCGAAACCGATTGTGTCC | More axillary branching 2 gene |
| AtMAX3 | AT2G44990 | CGTTGGTGAGCCCATGTTTG | TCCACCGAAACCGCATACTC | More axillary branching 3 gene |
| AtMAX4 | AT4G32810 | TATCGGGTCGTGAGGATGGA | GCAAACGAATGGACCCAACC | More axillary branching 4 gene |
| Genes involved in Auxin polar transport (efflux carrier) and controlling shoot branching | ||||
| AtPIN1 | AT1G73590 | ACGACAACCAGTACGTGGAG | TATGTTGTTCCCACCGTCCG | PIN-FORMED (PIN) protein encoding gene1 |
| AtPIN3 | AT1G70940 | TGGCCATGATCCTCGCTTAC | CGAAGATGGCGACAAAACGG | PIN-FORMED (PIN) protein encoding gene 3 |
| AtPIN7 | AT1G23080 | AGCCATGATCCTCGCTTACG | AGAGGGACGGCGAAAATAGC | PIN-FORMED (PIN) protein encoding gene 7 |
| Cytokinin biosynthetic pathway genes | ||||
| AtIPT5 | AT5G19040 | CGACGGAGGTTTTTCTCCGA | GAACTTTTCGACGGCGAGTG | Isopentenyl transferase encoding gene 5 |
| AtIPT7 | AT3G23630 | GACGCCACTGAGGTGTTCTT | CGACGATTCTCTCGCTTGGT | Isopentenyl transferase encoding gene 7 |
| Genes involved in Gibberellic acid biosynthesis pathway | ||||
| AtGA2ox1 | AT1G78440 | CTCGTTGCCCAAGTCAGAGA | TACTCAACCCAACCCACGTC | Gibberellic acid 2 oxidase 1 |
| AtGA20ox1 | AT4G25420 | GTGAGAGTGTTGGCTACGCA | CTCATGTCGTCGCAAAACCG | Gibberellic acid 20 oxidase 1 |
| AtGA20ox2 | AT5G51810 | TGGCCAGACGAAGAGAAACC | TTGACGACGAGGAAGAAGCC | Gibberellic acid 20 oxidase 2 |
| AtAXR1 | AT1G05180 | CGGACAGATTTGCTGCCAAC | ATCTGGGAGTACTGAGCCGT | Arabidopsis Auxin repressor 1 |
| AtRGA1 | AT2G01570 | TTGTCCAACCACGGGACTTC | AGCTCGTCGTCCATGTTACC | Arabidopsis Repressor of GA 1 |
| Arabidopsis housekeeping gene | ||||
| AtACT2 | AT3G18780 | AGGTTCTGTTCCAGCCATC | TTAGAAGCATTTCCTGTGAAC | Arabidopsis Actin coding gene 2 |