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. 2021 Feb 20;11(2):539. doi: 10.3390/nano11020539

Figure 1.

Figure 1

Cell proliferation of RAW 264.7 cells cultured with BSA-MWNTs. MWNTs suspended in a 0.10 mg/mL BSA working solution were mixed with an equal volume of 2X-concentrated medium to produce MWNT concentrations shown on the x-axes of the graphs. An equivalent number of cells were seeded in 48-well plates and incubated at 37 °C under standard cell culture conditions for 24 h prior to the experiment. Cell proliferation after incubation with control and test media for 24 h at 37 °C was determined by the crystal violet assay as described in the Methods, where the proliferation of control cells exposed to BSA in the absence of MWNTs was set to 100%. (Top) RAW 264.7 macrophage cell proliferation post 24-h incubation with various concentrations of BSA-pMWNTs. (Bottom) RAW 264.7 macrophage cell proliferation post 24-h incubation with various concentrations of BSA-cMWNTs. Both data sets are the mean of quadruple samples in three independent experiments ± the standard deviation (SD).