Figure 2.

CypA is released by cultured murine proximal tubular MCT cells under hypoxia/reoxygenation. MCT cells were cultured for 24 h in a hypoxic atmosphere, and then cells were reoxygenated for the indicated periods of time (A) Cell viability was assessed by the MTT assay. Mean ± SD of three independent experiments. *** p < 0.001 vs. normoxia 8 h; ### p < 0.001 vs. normoxia 24 h. (B,C) Western blot of CypA in cell supernatants (B) and in cellular extracts (C). Quantification and representative image. Mean ± SD of three independent experiments. * p < 0.05; ** p < 0.01. Uncropped gel scans shown in Figure S1B.