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. 2021 Feb 20;13(4):888. doi: 10.3390/cancers13040888

Figure 2.

Figure 2

Genetic deletion of miR-21 protects against breast tumor formation. In the orthotopic model, 1 × 106 MMTV-PyMT cells were injected into the mammary fat pad of miR-21+/+, miR-21+/− and miR-21−/− (n = 10 mice/group). (A) miR-21 expression in mammary fat pads of miR-21+/+, miR-21+/− and miR-21−/−, determined by one-way ANOVA and represented as mean ± SEM, (****—p < 0.0001, n = 2 mice/group; ●—Individual replicates of miR-21+/+ tumors, ▪—Individual replicates of miR-21+/− tumor, ▲—Individual replicates of miR-21−/− tumors). (B) Schema. (C) Tumor formation was noted in all miR-21+/+ and 45% of miR-21+/− mice, but the miR-21−/− had 0% tumor formation at 150 days determined by Mantel–Cox test (p < 0.001, n = 10 mice/group).