Figure 6.

Membrane permeability of biofilm cells. C. albicans biofilms formed after a 24 h incubation with CBD were incubated with PI. A–E. PI-stained biofilms were observed by CLSM. PI staining of untreated control (A); biofilms treated with 3.12 μg/mL (B), 12.5 μg/mL (C), 50 μg/mL (D), or 100 μg/mL (E) CBD. Magnification 100×. (F). Relative PI fluorescence was measured in a plate reader. The ratios between dead cells and metabolically active cells in biofilms was calculated as PI fluorescence values divided on values obtained from MTT assay (PI/MTT ratio). Data are presented as a fold increase in PI/MTT ratio in treated biofilms compared to PI/MTT ratio in untreated biofilms. * Significantly lower than the untreated control (p < 0.05).