Figure 7.

Up-regulation of miR-296-5p inhibited the expression of STAT3 in CRC cells. (A and B) The qRT-PCR experiment showed that miR-296-5p mimic up-regulated the mRNA level of miR-296-5p. U6 played the role of internal reference. (C and D) The qRT-PCR experiment showed that miR-296-5p mimic suppressed the mRNA level of STAT3, while overexpression of STAT3 reversed this effect. GAPDH played the role of internal reference. (E–G) The Western blot experiment showed that miR-296-5p mimic inhibited the protein level of STAT3, while over-expression of STAT3 reversed this effect. GAPDH played the role of internal reference. All experiments were repeated three times to obtain average values. ***p<0.001 vs MC+NC; ^{^^^}p<0.001 vs M+NC; ^###p<0.001 vs MC+STAT3.

Abbreviation: STAT3, signal transducer and activator of transcription 3.