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Detection of input clonal sequences and recombinants in HT-SGS validation experiments.
| Input wt:2M ratio | Count (%) of single-genome consensus seqs detected | ||
|---|---|---|---|
| wt | 2M | Recombinant | |
| wt only | 84 (100) | 0 | 0 |
| 2M only | 0 | 162 (100) | 0 |
| 1:1 | 52 (37.7) | 86 (62.3) | 0 |
| 1:5 | 24 (13.6) | 153 (86.4) | 0 |
| 5:1 | 89 (84.8) | 16 (15.2) | 0 |
| 1:50 | 2 (1.2) | 162 (98.8) | 0 |
| 50:1 | 128 (97.7) | 3 (2.3) | 0 |
