Table II.
Sample | N1 RNA (copies/mL) | N2 RNA (copies/mL) | cDNA copies recovereda | Input cDNA copies (SGS) | SGS recovered | SGS % recovery |
---|---|---|---|---|---|---|
Pt.1 (d9) | 3,069,099 | 2,832,963 | 24,233 | 8,220 | 1,276 | 15.5 |
Pt.1 (d11) | nd | 19,576 | 10,000 | 882 | 8.8 | |
Pt.1 (d13) | 314 | 386 | 124 | 124 | 16 | 12.9 |
Pt.1 (d15) | 13,470 | 11,105 | 1,807 | 1,807 | 284 | 15.7 |
Pt.1 (d17) | 3,774 | 2,919 | 70 | 70 | 12 | 17.2 |
Pt. 2 (d12) | 116,508 | 108,586 | 536 | 536 | 70 | 13.1 |
Pt.3 (d17) | nd | 17,531 | 10,000 | 1,210 | 12.1 | |
Pt. 4 (d8) | 872,984 | 841,366 | 605 | 605 | 108 | 17.9 |
Pt. 5 (d8) | 2,669,500 | 2,520,722 | 4,060 | 3,400 | 367 | 10.8 |
Pt. 6 (d8) | 105,735 | 92,156 | 255 | 255 | 31 | 12.2 |
Pt. 7 (d16) | 101,327 | 96,916 | 50 | 50 | 13 | 26.0 |
Sample volumes used for extraction were 140 μL ~ 300 μL.