Figure 3.

Incorporated tPA increased in the thrombus head. (A) Fibrinolysis was measured as fluorescence released in the bathing fluid from heads and tails in the absence or presence of 20 or 40 ng/mL of incorporated tPA. Inset shows the fluorescence released at 0 ng/mL of tPA on a smaller scale. (B) tPA, (C) PAI-1 and (D) plasminogen antigen concentrations were measured by ELISA in extracts of heads and tails. (E) Detection of tPA by zymography showing tPA and tPA–PAI-1 in extract of thrombus head and tail sections formed with incorporation of 0, 20 or 40 ng/mL tPA. Data shown are mean ± SEM (n = 3). *** p < 0.001 vs. no tPA.