Table 4.
Antibodies and procedures for immunohistochemical and histochemical staining.
| Immunohistochemical Staining | |||||||
|---|---|---|---|---|---|---|---|
| Primary Antibodies | |||||||
| Antigen | Company | Cat # | Antigen Retrieval | Dilution | Incubation | Species | Control Tissue |
| αSMA | Abcam | ab5694 | CB, 100 °C | 1:100 | ON, 4 °C | Rabbit | Blood vessel |
| ALDH1A1 | Abcam | ab52492 | CB, 100 °C | 1:800 | ON, 4 °C | Rabbit | Kidney, lung |
| Caveolin-1 | Abcam | ab32577 | CB, 100 °C | 1:750 | ON, 4 °C | Rabbit | Liver, lung |
| CD133/1 | Miltenyi Biotec | 130–108-062 | CB, 100 °C | 1:100 | ON, 4 °C | Mouse | Gallbladder |
| CD44 | Dako | M7082 | CB, 100 °C | 1:200 | ON, 4 °C | Mouse | Gallbladder |
| Col Iα1 | LifeSpan BioSciences | LS-B5932 | CB, 100 °C | 1:200 | ON, 4 °C | Mouse | Prostate |
| Col III | Abcam | ab7778 | CB, 100 °C | 1:300 | ON, 4 °C | Rabbit | Testis |
| Fibronectin | Abcam | ab6328 | TEB, 100 °C | 1:300 | ON, 4 °C | Mouse | Granulation tissue |
| ITGα2 | Abcam | ab133557 | CB, 100 °C | 1:1000 | ON, 4 °C | Rabbit | Testis |
| ITGα5 | Abcam | ab150361 | CB, 100 °C | 1:200 | ON, 4 °C | Rabbit | Placenta |
| ITGβ1 | ThermoFisher | MA5-17103 | CB, 100 °C | 1:200 | ON, 4 °C | Mouse | Small intestine |
| Ki67 | Dako | M7240 | CB, 100 °C | 1:1000 | ON, 4 °C | Mouse | Small intestine |
| MMP14 | Abcam | ab51074 | TEB, 100 °C | 1:1600 | ON, 4 °C | Rabbit | Placenta |
| Secondary Antibodies | |||||||
| Company | Cat # | Antigen Retrieval | Dilution | Incubation | Detection | Control Tissue | |
| Immpress HRP Anti-Rabbit IgG | Vector Labs | MP-7401 | - | Ready to use | 1 h, RT | DAB Peroxidase substrate kit (SK-4100) | - |
| Immpress HRP Anti-Mouse IgG | Vector Labs | MP-7402 | - | Ready to use | 1 h, RT | DAB Peroxidase substrate kit (SK-4100) | - |
| Histochemical Staining | |||||||
| Hyaluronan | Merck Millipore | 385911 | CB, 60 °C | 1:100 | 1 h, RT | ABC-AP-Kit (Vector Labs, AK-500) and Liquid Permanent Red (Dako, K0640) | Cartilage |
| Picrosirius red | Histolab | HL27150.0500 | - | Ready to use | 10 min, RT | Tendon | |
CB, Citrate buffer (pH 6); TEB, Tris/EDTA buffer (pH 9). Abcam, Cambridge, UK; Histolab, Askim, Sweden; LifeSpan Biosciences, Seattle, WA, USA; Merck Millipore, Burlington, MA, USA; Miltenyi Biotec, Bergisch Gladbach, Germany; Thermo Fisher Scientific, Waltham, MA, USA; Vector Labs, Burlingame, CA, USA; Dako: Agilent Technologies, Glostrup, Denmark; ThermoFisher: Waltham, MA USA.