Table 1.

Overview of A. fumigatus genotyping approaches and their features.

Typing Technique	Principle	Discriminatory Power 1	Advantage(s)	Disadvantage(s)	Reference(s)
TRESP	hypervariable TRs 2 in 3 genes	>0.997	specialist equipment not required ease of interpretation, reproducibility	lower resolution	[44]
	including cyp51A resistance markers	0.890
TRESPERG	hypervariable TRs in 4 genes	>0.997			[46]
	including cyp51A resistance markers	>0.993
TRESPERG + STRAf	hypervariable TRs in 4 genes plus 9 STRs 3 and cyp51A resistance markers	>0.999	high-resolution fingerprinting	technical expertise specialist equipment	[46]
STRAf	9 STRs	>0.999	high-resolution fingerprinting	technical expertise specialist equipment reproducibility interlaboratory variation	[30]
nSTRAf	8 STRs	0.9997	high-resolution fingerprinting single multiplex reaction longer, more accurate STRs	technical expertise specialist equipment	[40]
STRAf+	9 STRs and cyp51A resistance markers	na 4	high-resolution fingerprinting public database (AfumID)	technical expertise specialist equipment	[43]
NGS/WGS 5	genome	>0.999	maximum resolution	technical expertise specialist equipment bioinformatics capabilities	[65,66]

¹ Based on Simpson’s diversity index. ² TR, tandem repeat. ³ STR, short tandem repeat polymorphism microsatellite markers; also referred to as microsatellite length polymorphisms (MLP) or variable number of tandem repeats (VNTR). ⁴ na, not available. ⁵ NGS, next-generation sequencing; WGS, whole-genome sequencing.