Vaccinated and non-vaccinated birds shed virus after NDV challenge in the different experiments. (A) Oropharyngeal (OP) and cloacal (CL) swabs from birds in the control group and in groups vaccinated with adeno-F at high and low doses were tested by RRT-PCR after 2 and 4 days post-challenge (dpc). (B) Virus shedding from vaccinated birds with LaSota and adeno-F vaccines and non-vaccinated birds in OP and cloacal swabs at 2, 4, and 7 days after challenge with CA02, EG14, or TZ12. Viral titers in OP or CL swab samples from the different treatments (control, adeno-F—low and high doses, and LaSota) on the same day and with the same NDV challenge strain. The mean ± standard errors with different letters (a, b, or c) are statistically significant (p < 0.05). CL: cloacal swab; OP: oropharyngeal swab.