NBMI treatment does not affect intra-worm Hg level. a–c Expression of aat genes in worms treated with 60 μM MeHg or 100 μM NBMI, or pre-treated with 100 μM NBMI for 2 h, then treated 60 μM MeHg for 1 h in NBMI-free buffer. d Intra- worm Hg content of worms treated with MeHg or NBMI. Data are expressed as mean ± SD of at least three biological replicates. e
p values of two-way ANOVA analysis. Comparisons were made by two-way ANOVA and Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ***P < 0.001 as compared with untreated control. #P < 0.05, ##P < 0.01, and ###P < 0.001 as compared with the group with NBMI treatment alone. The horizontal bars represent a statistical significant difference between the groups