Table 2. List of primers used for PCR analysis.
| Primer name | Sequence (5′ to 3′) | Size of amplicon (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
| Ehist-18S-S | GTTTTATACATTTTGAAGACTTTATG | 434 | 60 | [23] |
| Ehist-18S-AS | CAGATCTAGAAACAATGCTTCTCT | [23] | ||
| Edisp-18S-S | ATTTTATACATTTTGAAGACTTTACATT | 458 | 60 | [23] |
| Edisp-18S-AS | GAACAAGGTAGTATTGATATACTTG | [23] | ||
| Enut-18S-S | TTTTATACATTTTGAAGACTTTGCATA | 452 | 60 | [23] |
| Enut-18S-AS | AAGGTAATATTGATATACTCAGATTA | [23] | ||
| Ecoli-18S-S | GAATGTCAAAGCTAATACTTGACG | 160 | 60 | [24] |
| Ecoli-18S-AS | GATTTCTACAATTCTCTTGGCATA | [24] | ||
| Echattoni1 | AGGATTTGTTTTATAACAAGTTC | 215 | 55 | [25] |
| Echattoni2 | TAAATAACCTTTCTCCTTTTTCTATC | [25] | ||
| Epolecki1 | TCGATATTTATATTGATTCAAATG | 201 | 55 | [25] |
| Epolecki2 | CCTTTCTCCTTTTTTTAT ATTAG | [25] | ||
| Emoshkov3 | TGACGACAAATAACTCTCGAGG | 200 | 60 | Present study |
| Emoshkov4 | GCCTTCAAAATGATTAAAACCACC | Present study | ||
| D-A5 (EhR1) | CTGGTTAGTATCTTCGCCTGT | 364–540a | 58 | [27] |
| D-A3 (EhR2) | GCTACACCCCCATTAACAAT | [27] |
aSize range obtained in this study.