Fig 6. G4 structures are detectable in adult schistosomes.

Male and female worms were stained with anti-quadruplex recognising BG4 (green) and counterstained with DAPI (blue). +/+ denotes the use of both primary and secondary antibodies, -/+ denotes the use of secondary antibody only (control). Z stacks of adult anterior (Ant), midsection (Mid) and posterior (Post) regions were taken using a Leica SP8 confocal microscope. Samples were imaged at 20X (scale bar = 50 μm) and then 100X, (scale bar = 10 μm), with box denoting region enlarged. Strong BG4 signal (488 nm) is co-localised with DAPI signal (405 nm) and is observed in both female and male samples.