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. Author manuscript; available in PMC: 2021 Mar 2.
Published in final edited form as: J Pineal Res. 2019 Oct 7;67(4):e12610. doi: 10.1111/jpi.12610

FIGURE 4.

FIGURE 4

Actions of melatonin on altered tyrosinase activity due to selected inhibitors in MNT-1 melanoma cell line. Applied 10−3 mol/L PTU (A) or 200 μmol/L kojic acid (B) significantly affected tyrosinase activity compared to co-incubated cells with melatonin for 72 hours. Briefly, cells were lysed with 0.5% Triton® X-100, centrifuged at 16 000 g (15 min, 4°C), 5 mmol/L L-DOPA was added to resultant supernatant, and the dopachrome formation was evaluated by measuring absorbance at λ = 475 nm. Data were presented as the mean + SD (n = 5), the values are expressed as percentage of the control sample. Statistically significant differences were indicated as *P < .05, **P < .01, ***P < .001 while comparison of PTU- or kojic acid-treated cells versus control sample was indicated as ###P < .001