Skip to main content
. Author manuscript; available in PMC: 2021 Mar 2.
Published in final edited form as: Neuron. 2020 May 5;107(1):65–81.e9. doi: 10.1016/j.neuron.2020.03.031

Figure 1. Galc Is Efficiently Ablated in Schwann Cells of Conditional Knockout Mice.

Figure 1.

(A–C) Dose-dependent reduction of GALC in P35 sciatic nerves from Galc(+/+), Galc(+/−), and Galc(−/− ) mice.

(A) Galc mRNA expression, normalized to β-Actin, and reported relative to average WT expression.

(B) GALC enzymatic activity, measured as nmol of fluorogenic substrate MUGAL, normalized to protein and time.

(C) GALC immunofluorescence in longitudinal sections of P35 sciatic nerves. Polyclonal anti-GALC antibody (green); DAPI (blue).

(D–I) Efficient Galc ablation in Schwann cells of conditional knockout mice.

(D and E) Galc mRNA expression of desheathed P35 sciatic nerves (D) and primary Schwann cells (E).

(F and G) GALC activity of desheathed P35 sciatic nerves (F) and primary Schwann cells (G).

(H) GALC immunofluorescence and quantification of teased fibers from P14 sciatic nerves. GALC (green); LAMP1 (red); DAPI (blue). (I) Higher magnification of (H).

(J) Psychosine measured by HPLC-MS from P35 sciatic nerves.

Scale bars, 25 μm (C), 30 μm (H), and 14 μm (I). Error bars represent mean ± SEM, n = 3 biological replicates and 3 technical replicates per experiment (n = 6 for J). Statistical significance was calculated by one-way ANOVA (A, B, D, F, and J) or Student’s t test (E, G, and H). In all figures, asterisks represent statistical significance (*p < 0.05, **p < 0.01; ***p < 0.005, ****p < 0.001). n.s., not significant.