KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-CASPR | A gift from Elior Peles, Weizmann Institute. (Peles et al., 1997) | #A6061 RRID:AB_231422 0 |
| Mouse anti-APC (CC-1) | Millipore | Millipore Cat# OP80, RRID:AB_2057371 |
| Mouse anti-CD-68 | Dako (Agilent) | Agilent Cat# M0814, RRID:AB_2314148 |
| Rabbit anti-CD206 | Proteintech | Cat# 18704–1-AP RRID:AB_105972 32 |
| Goat anti-Choline Acetyltransferase (ChAT) | Millipore | Millipore Cat# AB144P, RRID:AB_2079751 |
| Rat anti-mouse F4/80 | Bio-Rad | Bio-Rad Cat# MCA497GA, RRID:AB_323806 |
| Chicken anti-GALC | A gift from Chris Lee and Christopher Eckman. (Lee et al., 2010) | CL1021AP |
| Rabbit anti-GAPDH | Sigma-Aldrich | Sigma-Aldrich Cat# G9545, RRID:AB_796208 |
| Rat anti-HA High Affinity | Roche | Roche Cat# 11867423001, RRID:AB_390918 |
| Anti-HA-Peroxidase, High Affinity | Roche | Roche Cat# 12013819001, RRID:AB_390917 |
| Rabbit anti-IBA1 (for immunocytochemistry) | Wako Chemicals | Cat# 019–19741 RRID:AB_839504 |
| Rabbit anti-Kv1.1 (KCNA1) | Alomone Labs | Cat# APC-009, RRID:AB_204014 4 |
| Rabbit anti-LAMP-1 | Abeam | Abcam Cat# ab24170, RRID:AB_775978 |
| Chicken anti-MPZ | Aves | Aves Labs Cat# PZO, RRID:AB_2313561 |
| Rabbit anti-MPZ | Abeam | Abcam Cat# ab31851, RRID:AB_2144668 |
| Mouse anti-Nav1.6 clone K87A/10 | UC Davis / NeuroMab | RRID:MMRRC_06 5966-UCD |
| Mouse anti-Pan-Neurofascin L11A/41 | Millipore | Millipore Cat# MABN621 RRID: AB_10672370 |
| Anti-galactocerebroside (O1) | A gift from Pablo Paez, SUNY Buffalo. (Sommer and Schachner, 1981) | O1 Hybridoma |
| Rabbit anti-human OLIG2 | Proteintech | Proteintech Cat# 13999–1-AP, RRID:AB_2157541 |
| Rabbit anti-PMP2 | Proteintech | Proteintech Cat# 12717–1-AP, RRID:AB_2166978 |
| Rabbit anti-PU.1 (Spi-1) (T-21) | Santa Cruz | Santa Cruz Biotechnology Cat# sc-352, RRID:AB_632289 |
| Mouse anti-Tubulin β3 (Tuj1) | Biolegend | Covance Cat# MMS-435P, RRID:AB_2313773 |
| Rabbit anti-β-Tubulin | Novus | Novus Cat# NB600–936, RRID:AB_1000065 6 |
| Brilliant Violet 711 anti-mouse/human CD11b antibody (M1/70) | BioLegend | Cat# 101241 RRID:AB_112187 91 |
| PE anti-mouse Ly-6G antibody (1A8) | BioLegend | Cat# 127607 RRID:AB_118610 4 |
| APC anti-mouse F4/80 antibody (BM8) | BioLegend | Cat# 123115 RRID:AB_ 893493 |
| PE/Cy7 anti-mouse Ly-6C antibody (HK1.4) | BioLegend | Cat# 128017 RRID:AB_ 1732093 |
| FITC Hamster Anti-Mouse CD11C (HL3) | BD PharMingen | Cat# 553801 RRID:AB_395060 |
| APC/Cy7 anti-mouse CD86 antibody (GL-1) | BioLegend | Cat# 105029 RRID: AB_2074993 |
| PerCP anti-mouse I-A/I-E antibody (M5/114.15.2) | BioLegend | Cat# 107623 RRID: AB_893586 |
| Brilliant Violet 510 anti-mouse/human CD11b antibody (M1/70) | BioLegend | Cat# 101245 RRID:AB_256139 0 |
| APC/Cyanine7 anti-mouse F4/80 antibody (BM8) | BioLegend | Cat# 123117 RRID: 893489 |
| iNOS Monoclonal antibody (CXNFT), APC, eBioscience | Thermo Fisher Scientific | Cat#17-5920-82 RRID:AB_257324 4 |
| Human/Mouse Arginase 1/ARG1 Fluorescein- conjugated antibody | R&D Systems | Cat#IC5868F RRID:AB_107181 18 |
| PE/Cy7 anti-mouse CD206 (MMR) antibody (C068C2) | BioLegend | Cat# 141719 RRID:AB_256224 7 |
| EGR2 Monoclonal Antibody (erongr2), PE, eBioscience | Thermo Fisher Scientific | Cat# 12-6691-82 RRID:AB_107178 04 |
| Brilliant Violet 711 anti-mouse TNF-α antibody (MP6-XT22) | BioLegend | Cat# 506349 RRID:AB_262980 0 |
| Brilliant Violet 421 anti-human IL-10 antibody (JES3–9D7) | BioLegend | Cat# 501421 RRID:AB_108969 47 |
| PerCP/Cy5.5 anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) antibody (C15.6) | BioLegend | Cat# 505211 RRID:AB_256622 4 |
| TruStain FcX (anti-mouse CD16/32) antibody (93) | BioLegend | Cat#101319 RRID:AB_157497 3 |
| Alexa Fluor 488-AffiniPure F(ab’)2 Fragment Donkey anti-Chicken IgY | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 703-546-155, RRID:AB_2340376 |
| Rhodamine Red-X-AffiniPure Donkey Anti-Rabbit IgG | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 711-295-152, RRID:AB_2340613 |
| Alexa Fluor 647 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 711-606-152, RRID:AB_2340625 |
| Goat anti-Mouse lgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A- 21145, RRID:AB_2535781 |
| Alexa Fluor 594-AffiniPure Goat Anti-Mouse IgG, Fc subclass 2a Specific antibody | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 115-585-206, RRID:AB_2338886 |
| Cy3-AffiniPure Donkey Anti-Goat IgG (H+L) antibody | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 705-165-003, RRID:AB_2340411 |
| Alexa Fluor 488-AffiniPure Donkey Anti-Rat IgG antibody | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 712-545-153, RRID:AB_2340684 |
| Rhodamine (TRITC)-AffiniPure Donkey Anti-Rat IgG antibody | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 712-025-153, RRID:AB_2340636 |
| Donkey Anti-Rabbit Rabbit IgG (H+L) Polyclonal, HRP-Conjugated antibody | Novus | Novus Cat# NB 7185, RRID:AB_524677 |
| Peroxidase-AffiniPure Donkey Anti-Chicken IgY (IgG) antibody | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 703-035-155, RRID:AB_1001528 3 |
| Peroxidase-AffiniPure Goat Anti-Rat IgG, Light Chain Specific | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 112-035-175, RRID:AB_2338140 |
| Goat anti-Mouse IgM, mu chain, FITC conjugate antibody, Millipore | Millipore | Millipore Cat# AP128F, RRID:AB_1121579 1 |
| Bacterial and Virus Strains | ||
| DH5a | Thermo Fisher Scientific | Catalog number: 1825801 2 |
| Biological Samples | ||
| Mouse Nerve RNA | This paper | N/A |
| Mouse Schwann cell RNA | This paper | N/A |
| Mouse BMDM RNA | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2,2,2-tribromoethanol (avertin) | Millipore Sigma | Cat#T48402 |
| 4-methylumbelliferone β-D-galactopyranoside | Sigma-Aldrich | Cat#M1633 |
| 4-methylumbelliferone sodium salt | Sigma-Aldrich | Cat#M1508 |
| Amicon Ultra-15 | Fisher Scientific | Cat#UFC901024 |
| C8 galactosyl ceramide | Avanti Polar Lipids | Cat#860538P |
| Diethyl pyrocarbonate (DEPC) | Fisher Scientific | Cat#FERR0601 |
| D-Mannose 6-phosphate | Sigma-Aldrich | Cat#M6876 |
| DNA 100bp ladder | Goldbiotechnology | Cat#D001–2500 |
| Pierce ECL Western Blotting | Thermo Fisher Scientific | Cat#32106 |
| Pierce ECL Plus Western Blotting | Thermo Fisher Scientific | Cat#32132 |
| GE Healthcare Amersham | Fisher Scientific | Cat#45-001-507PM |
| HisPur Cobalt Resin | Thermo Fisher Scientific | Cat#89964 |
| Imidazole | Millipore Sigma | Cat#T6330 |
| β-Mercaptoethanol | Sigma-Aldrich | Cat#M6250 |
| Phosphatase Cocktail 2 | Sigma-Aldrich | Cat#P5726 |
| Phosphatase Cocktail 3 | Sigma-Aldrich | Cat#P0044 |
| PMSF | Sigma-Aldrich | Cat#45-P7626 |
| Precision Plus Protein Dual Color Standards | Bio-Rad | Cat#161–0394 |
| Psychosine (Galactosyl Sphingosine, d18:1) | Avanti Polar Lipids | Cat#860537P |
| Deuterated Psychosine Quantitative Mass Spec Standard | Avanti Polar Lipids | Cat#330714W |
| Saponin | Sigma-Aldrich | Cat#47036 |
| Thermo Scientific Pierce Tissue Strainers (250 microns) | Fisher Scientific | Cat#87791 |
| Vectashield Antifade Mounting Medium | Vector Labs | Cat#H-1000 |
| Vetabond reagent for tissue section (TESPA) | Vector Labs | Cat#SP-1800 |
| B27 Supplement | Thermo Fisher Scientific | Cat#17504–044 |
| Collagen | Corning | Cat#354236 |
| Collagenase type I | Fischer Scientific | Cat#NC9633623 |
| Dispase II | Sigma-Aldrich | Cat#D4693 |
| Forskolin | EMD Millipore | Cat#344270 |
| Human Recombinant Laminin 211 | Biolamina | Cat#LN211–03 |
| Lipofectamine 3000 | Thermo Fisher Scientific | Cat#L3000008 |
| Recombinant Human Nrg1-β1 | R&D Systems | Cat#396-HB-050 |
| Recombinant Mouse M-CSF | Biolegend | Cat#576404 |
| RPMI-1640 medium | Thermo Fisher Scientific | Cat#21870076 |
| Nerve Growth Factor NGF | Envigo | Cat#BT.5017 |
| Opti-MEM | Thermo Fisher Scientific | Cat#11058–021 |
| Critical Commercial Assays | ||
| Cell counting kit-8 | Dojindo | Cat#CK04–05 |
| Cholesterol Assay Kit (aka Filipin) | Abeam | Cat#Ab133116 |
| Fixation/Permeabilization Solution Kit | BD Cytofix/Cytoperm | Cat#554714 |
| Reverse Transcriptase (superscript III Kit) | Thermo Fisher Scientific | Cat#18080–051 |
| Taqman Gene Expression Assay: 18S (Hs99999901_s1) | Thermo Fisher Scientific | Cat#4331182 |
| Taqman Gene Expression Assay: Ddit3/CHOP (Mm00492097_m1) | Thermo Fisher Scientific | Cat#4331182 |
| Taqman Gene Expression Assay: XBP1 (Mm03464496_m1) | Thermo Fisher Scientific | Cat#4331182 |
| Taqman Gene Expression Assay: HSPa5/BiP (Hs99999174_m1) | Thermo Fisher Scientific | Cat#4331182 |
| Taqman Gene Expression Assay: MRC1/CD206 (Mm01329362_m1) | Thermo Fisher Scientific | Cat#4331182 |
| Experimental Models: Cell Lines | ||
| Human: Dermal Fibroblasts | Telethon biobank (Shin et al., 2016) | N/A |
| Mouse: Primary Schwann cells | This paper | N/A |
| Mouse: Primary bone marrow derived macrophages | This paper | N/A |
| Mouse: Primary embryonic fibroblasts | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.C-Tg(GALCfl/fl) | This paper. (Weinstock et al., 2020) | N/A |
| Mouse: B6.CE-Galctwi/J | The Jackson Laboratory | Cat#000845 |
| Mouse: B6.C-Tg(CMV-cre)1Cgn/J | The Jackson Laboratory | Cat#006054 |
| Mouse: B6N.FVB-Tg(Mpz-cre)26Mes/J (aka PO Cre) | The Jackson Laboratory | Cat#017927 |
| Mouse: B6.129P2-Lyz2tm1(cre)Ifo/J (aka LysM Cre) | The Jackson Laboratory | Cat#004781 |
| Mouse: B6;129S6-Gt(ROSA)26Sortm14(CAG-dTomato)Hze/J (aka tdTomato) | The Jackson Laboratory | Cat#007908 |
| Mouse: B6.Cg-Tg(Thy1-YFP)HJrs/J | The Jackson Laboratory | Cat# 003782 |
| Mouse: ATF3-GFP | Y.-C.C. and C. Woolf unpublished data | N/A |
| Oligonucleotides | ||
| Primers for qPCR, See Table S2 | This paper | N/A |
| Primers for cloning hGALC-6xHis-HA, see Table S2 | This paper | N/A |
| Recombinant DNA | ||
| hGALC-HA | (Shin et al., 2016)) | N/A |
| hGALC-6xHis-HA | This paper | N/A |
| Software and Algorithms | ||
| ImageJ | (Schneider et al., 2012) | https://imagej.net/Welcome |
| Graph Pad Prism 7 | GraphPad | Graphpad.com |