Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Saccharomyces cerevisiae) |
PIC2 | Saccharomyces Genome Database |
SGD:S000000855 | |
| Gene (Saccharomyces cerevisiae) |
MIR1 | Saccharomyces Genome Database |
SGD:S000003838 | |
| Gene (Mus musculus) |
SLC25A3 | doi:10.1074/jbc.RA117.000265 | Ensembl: ENSMUSG00000061904 |
|
| Strain, strain background (Saccharomyces cerevisiae) |
BY4741 | Thermo Scientific | ||
| Strain, strain background (Lactococcus lactis) |
NZ9000 pepN::nisRK | doi:10.1007/s00253-005-0107-6 | ||
| Strain, strain background (Escherichia coli) |
BL21(DE3) | New England Biolabs | Cat# C2527H | |
| Transfected construct (Mus musculus) |
SLC25A3 | doi:10.1074/jbc.RA117.000265 | ||
| Cell line (Mus musculus) |
MEF | doi:10.1074/jbc.RA117.000265 | ||
| Antibody | SLC25A3 (rabbit polyclonal) |
This paper | See 'Materials and methods' for details WB(1:2000) |
|
| Antibody | PIC2 (rabbit polyclonal) |
doi:10.1074/jbc.M113.470674 | WB(1:1000) | |
| Antibody | COX1 (mouse monoclonal) |
Abcam | ab14705 | WB(1:2000) |
| Antibody | TOM40 (rabbit polyclonal) |
ProteinTech | 18409-1-AP | WB(1:2000) |
| Recombinant DNA reagent | EV | MoBiTec | Cat# ELV00200-01 | pNZ8148 expression vector containing the nisA promoter |
| Recombinant DNA reagent | WT; C21A; C29A; H33A; C44A; C225A; Q47A; V48A; Q86A; K90A; D124A; L127A; Q176A; G268A |
This paper | See Supplementary file 1 |
pNZ8148 with wild-type PIC2 sequence or with individual residue mutated |
| Recombinant DNA reagent | pHis.Parallel.1 | doi:10.1006/prep.1998.1003 | Expression vector for E. coli | |
| Chemical compound, drug | Nisin | MoBiTec | Cat# VS-ELK01000-02 |
|
| Chemical compound, drug | Silver | SPEX certiPrep | Lot #19-13 AGX | |
| Chemical compound, drug | Arsenate | Alfa Aesar | Lot #U21A031 | |
| Chemical compound, drug | Phen Green SK | Invitrogen | Lot #2034143 | |
| Software, algorithm | BioEdit | Ibis Biosciences, USA | RRID:SCR_007361 |
http://www.mbio.ncsu.edu/
BioEdit/bioedit.html |
| Software, algorithm | Coot | doi:10.1107/S0907444904019158 | RRID:SCR_014222 | |
| Software, algorithm | CD-HIT | doi:10.1093/bioinformatics/bts565 | RRID:SCR_007105 | |
| Software, algorithm | ConSurf server | doi:10.1093/nar/gkw408 | RRID:SCR_002320 | |
| Software, algorithm | Fiji (ImageJ) | doi:10.1038/nmeth.2019 | RRID:SCR_002285 | |
| Software, algorithm | HMMER, v3.3 | doi:10.1093/nar/gkr367 | RRID:SCR_005305 | http://hmmer.org |
| Software, algorithm | IQ-TREE, v2.0.3 | doi:10.1093/molbev/msu300 | RRID:SCR_017254 | http://www.iqtree.org/ |
| Software, algorithm | MEGA Software, v10.1.8 | doi:10.1093/molbev/msz312 | RRID:SCR_000667 | https://www.megasoftware.net/ |
| Software, algorithm | PHENIX | doi:10.1107/S2059798319011471 | RRID:SCR_014224 | https://www.phenix-online.org/ |
| Software, algorithm | Prism | Graph Pad, Inc | RRID:SCR_002798 | |
| Software, algorithm | PyMOL | Schrödinger, LLC | RRID:SCR_000305 | http://www.pymol.org/ |
| Software, algorithm | SWISS-MODEL | doi:10.1093/nar/gkg520 | RRID:SCR_018123 | https://swissmodel.expasy.org/ |
| Other | Bovine ADP/ATP carrier | doi:10.1038/nature02056 | PDB:1OKC | Crystal structure of the bovine ADP/ATP carrier |
| Other | Yeast ADP/ATP carrier, c-state |
doi:10.1073/pnas.1320692111 | PDB:4C9G | Crystal structure of the yeast ADP/ATP carrier in the cytoplasmic-open state |
| Other | Yeast ADP/ATP carrier, m-state |
doi:10.1016/j.cell.2018.11.025 | PDB:6GCI | Crystal structure of the yeast ADP/ATP carrier in the matrix-open state |