Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| gene (Mus musculus) | Esr1 | MGI | MGI:1352467 NCBI Gene: 13982 |
|
| Strain, strain background (Mus musculus) |
Esr1tm1Sakh
Esr1 floxed mouse line; CD-1;129P2 mixed background |
Feng et al., 2007 | MGI:4459300 | Targeted conditional mutation allele. Females used for experiments |
| Strain, strain background (Mus musculus) |
Tg(Nkx2-1-cre)2Sand
Nkx2-1Cre mouse line CD-1;129P2 mixed background |
Xu et al., 2008 | MGI: 3773076 | BAC transgenic Cre driver. Females used for experiments |
| Antibody | anti-ERα (mouse monoclonal) | Santa Cruz | Cat# sc-8002, RRID:AB_627558 | IF (1:250) |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | T5648 | |
| Software, algorithm | Seurat R package | Butler et al., 2018 | version 3.2.0 | Single-cell RNA sequencing analyses |
| Software, algorithm | Prism | GraphPad | version 8 | Physiology data analyses |
| Software, algorithm | VitalView software | Starr Life Sciences | version 5.1 | Core temperature and movement data collection |
| Software, algorithm | Mercury Analysis Software | Star:ODDI | version 5.7 | Tail temperature data collection |
| Other | DAPI stain | Invitrogen | D1306, RRID:AB_2629482 | IF (1 µg/mL) |