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. 2021 Feb 26;478(4):927–942. doi: 10.1042/BCJ20200596

Figure 5. Effects of pH and ligand binding on the nIR MCD Spectra of ferric H93K SWMb.

Figure 5.

(A) nIR MCD spectrum (580–1900 nm) of ferric WT SWMb (790 µM; blue trace) and ferric H93K SWMb at pH 7.0 (470 µM; solid red trace) and pH 9.5 (180 µM; dashed red trace). The product of the reaction of ferric H93K SWMb (490 µM;) with 10 mM imidazole at pH 7.0 is indicated by the black trace. (B) nIR MCD spectrum (580–1900 nm) of ferric WT SWMb (640 µM; green trace) and ferric H93K SWMb (428 µM; dark green trace) after reaction with of 100 mM nitrite.