Figure 2. Workflow of in-situ CCT product characterisation.

Frozen/thawed epidermis was incubated with [³H]HHA for up to 24 h, then the insoluble material (A) was treated with a sequence of extractants and enzymes, yielding a series of insoluble residues (B–H), some of which (B and H) were assayed for radioactivity. The material solubilised at each step (extracts a–g′) was also assayed for ³H.