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Trajectory segment analysis to correlate velocity and cellular density. (A) Diagram of trajectory segment analysis. (B) Example of randomly chosen long trajectory from E. faecalis optical-bottom-well biofilm. (C and D) Segment-by-segment plot of local cellular density (C) and velocity (D). (E) Example of randomly chosen long trajectory from S. Typhimurium biofilm. (F and G) Segment-by-segment plot of local cellular density (F) and velocity (G).
