FIG 8.

Mutation of the inverted repeat located within the hpdBCA upstream region leads to an inability to respond to p-HPA. Expression from the P_hpdB-phoZ reporter was assessed in the presence or absence of an inverted repeat (AAAAAG-N₁₃-CTTTTT) located 82 bp upstream of the hpdB start codon. Inverse PCR was used to delete the entire inverted repeat, including the spacer or the 5′ arm of the inverted repeat. Minimal medium was supplemented with 2 mg/ml p-HPA to determine whether any changes in expression were seen as a result of the mutation of the inverted repeat. ***, P < 0.001.