TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant feature(s) | Source or reference |
|---|---|---|
| C. difficile strains | ||
| 630Δerm | Erythromycin sensitive strain of 630 | 42 |
| 630Δerm PhpdB-SNAP | 630Δerm with plasmid PhpdB-SNAP | This study |
| 630Δerm Pfdx-SNAP | 630Δerm with plasmid Pfdx-SNAP | This study |
| 630Δerm PhpdB-gusA | 630Δerm with plasmid PhpdB-gusA | This study |
| 630Δerm PhpdB-phoZ | 630Δerm with plasmid PhpdB-phoZ | This study |
| 630Δerm Pfdx-phoZ | 630Δerm with plasmid Pfdx-phoZ | This study |
| CDIP217 | 630Δerm sigL::erm | 28 |
| 630Δerm sigL::erm PhpdB-SNAP | 630Δerm sigL::erm with plasmid PhpdB-SNAP | This study |
| Plasmids | ||
| PhpdB-SNAP | pMTL84151 plasmid carrying a SNAP-tag under the control of the hpdBCA promoter region | 26; this study |
| PhpdB-gusA | pMTL84151 plasmid carrying gusA under the control of the hpdBCA promoter | This study |
| PhpdB-phoZ | pMTL84151 plasmid carrying phoZ under the control of the hpdBCA promoter | This study |
| Pfdx-SNAP | pMTL84153 carrying SNAP-tag under control of the fdx promoter | This study |
| Pfdx-phoZ | pMTL84153 carrying phoZ under control of the fdx promoter | This study |
| PhpdB-SNAP P1mut | pMTL84151 plasmid carrying a SNAP-tag under the control of the hpdBCA promoter region with a mutation of the P1 site from TAT to TGC | This study |
| PhpdB-SNAP P2mut | pMTL84151 plasmid carrying a SNAP-tag under the control of the hpdBCA promoter region with a mutation of the P2 site from TAT to TGC | This study |
| PhpdB-SNAP P1mut P2mut | pMTL84151 plasmid carrying a SNAP-tag under the control of the hpdBCA promoter region with mutation in both the P1 and P2 sites from TAT to TGC | This study |
| PhpdB-phoZΔIR | 630Δerm with plasmid PhpdB-phoZ with the inverted repeat upstream of hpdB removed | This study |
| PhpdB-phoZΔ5′IR | 630Δerm with plasmid PhpdB-phoZ with the 5′ arm of the inverted repeat upstream of hpdB removed | This study |
| pMC358 | Plasmid carrying phoZ | 15 |
| pRPF185 | Plasmid carrying gusA | 25 |