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Examination of cell-free culture supernatants by SDS-PAGE. Cells from overnight-grown cultures were spun down, and the supernatants, after filtration and concentration, were analyzed by SDS-PAGE. Supernatant proteins were visualized after staining with Coomassie brilliant blue. Protein bands were quantified using Bio-Rad Quantity One software (amounts relative to that in the ΔtonB strain are shown). The key genetic characteristics of strains used are shown at the top.
