Genetic evidence that MapA can utilize the Lap secretion system. (A) Biofilm formation by strains in KA medium carrying mutations in adhesin genes and genes encoding the Map secretion system outer membrane pore component. (B) Biofilm formation in KA medium for strains carrying mutations in adhesin genes and genes encoding the Lap secretion system outer membrane pore component. For statistical tests, hypothesis testing was conducted in GraphPad Prism 8 using two-tailed t tests with Holm-Sidak’s multiple comparison correction. P values are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05.